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Thin Film Microextraction Enables Rapid Isolation and Recovery of DNA for Downstream Amplification Assays

https://doi.org/10.1021/acs.analchem.1c05380

Eitzmann, Derek R.; Varona, Marcelino; Anderson, Jared L. (March 2022, Analytical Chemistry)

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Advances in Mutation Detection Using Loop-Mediated Isothermal Amplification

https://doi.org/10.1021/acsomega.0c06093

Varona, Marcelino; Anderson, Jared L. (February 2021, ACS Omega)
null (Ed.)
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Sequence-Specific Detection of ORF1a, BRAF, and ompW DNA Sequences with Loop Mediated Isothermal Amplification on Lateral Flow Immunoassay Strips Enabled by Molecular Beacons

https://doi.org/10.1021/acs.analchem.0c05355

Varona, Marcelino; Eitzmann, Derek R.; Anderson, Jared L. (March 2021, Analytical Chemistry)
null (Ed.)
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Metal-containing and magnetic ionic liquids in analytical extractions and gas separations

https://doi.org/10.1016/j.trac.2021.116275

Varona, Marcelino; Eor, Philip; Ferreira Neto, Luiz Carlos; Merib, Josias; Anderson, Jared L. (July 2021, TrAC Trends in Analytical Chemistry)
null (Ed.)
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Modification of polyacrylate sorbent coatings with carbodiimide crosslinker chemistry for sequence-selective DNA extraction using solid-phase microextraction

https://doi.org/10.1039/D0AY00980F

Varona, Marcelino; Shimelis, Olga I.; Anderson, Jared L. (July 2020, Analytical Methods)

Selective DNA extraction is immensely useful for the isolation and detection of low-abundance sequences. Oligonucleotide-modified substrates are often used to capture sequences of interest for downstream analysis. In this study, we explore the chemical modification of commercial-available polyacrylate solid-phase microextraction fibers for selective DNA analysis using carbodiimide crosslinker chemistry. Reproducible modification conditions are found and the fibers were subsequently applied for selective DNA analysis. Several experimental parameters such as stir-rate, desorption time, and buffer-type are optimized. The developed method was able to selectively extract the target DNA sequence (260 bp) in the presence of 100-fold excess interfering salmon testes DNA.
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Magnetic Ionic Liquids as Solvents for RNA Extraction and Preservation

https://doi.org/10.1021/acsomega.0c01098

Zhu, Chenghui; Varona, Marcelino; Anderson, Jared L. (May 2020, ACS Omega)

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Nucleic acid extraction: Fundamentals of sample preparation methodologies, current advancements, and future endeavors

https://doi.org/10.1016/j.trac.2020.115985

Emaus, Miranda N.; Varona, Marcelino; Eitzmann, Derek R.; Hsieh, Shu-An; Zeger, Victoria R.; Anderson, Jared L. (September 2020, TrAC Trends in Analytical Chemistry)
null (Ed.)
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Visual Detection of Single-Nucleotide Polymorphisms Using Molecular Beacon Loop-Mediated Isothermal Amplification with Centrifuge-Free DNA Extraction

https://doi.org/10.1021/acs.analchem.9b01762

Varona, Marcelino; Anderson, Jared L. (May 2019, Analytical Chemistry)

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Sequence-specific preconcentration of a mutation prone KRAS fragment from plasma using ion-tagged oligonucleotides coupled to qPCR compatible magnetic ionic liquid solvents

https://doi.org/10.1016/j.aca.2019.04.005

Emaus, Miranda N.; Varona, Marcelino; Anderson, Jared L. (August 2019, Analytica Chimica Acta)

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Solid-Phase Microextraction Enables Isolation of BRAF V600E Circulating Tumor DNA from Human Plasma for Detection with a Molecular Beacon Loop-Mediated Isothermal Amplification Assay

https://doi.org/10.1021/acs.analchem.9b05323

Varona, Marcelino; Eitzmann, Derek R.; Pagariya, Darshna; Anand, Robbyn K.; Anderson, Jared L. (January 2020, Analytical Chemistry)

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